Structure - based functional analysis of plasmid R 6 K ’ s replication protein : 1 Key amino acids at the π / DNA interface
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چکیده
Structure-based functional analysis of plasmid R6K’s replication protein: 1 Key amino acids at the π/DNA interface 2 3 Selvi Kunnimalaiyaan, Sheryl A. Rakowski and Marcin Filutowicz* 4 5 Department of Bacteriology, University of Wisconsin 6 420 Henry Mall, Madison, Wisconsin 53706 7 8 9 10 11 12 13 14 Running title: Key amino acids for π binding and function 15 16 17 18 19 20 21 22 23 24 25 26 27 * Corresponding author. Mailing address: Department of Bacteriology, University of 28 Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706. Phone (608) 262-6947. Fax: (608) 29 262-9865. E-mail: [email protected] 30 31 32 AC CE PT ED Copyright © 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. J. Bacteriol. doi:10.1128/JB.00109-07 JB Accepts, published online ahead of print on 20 April 2007
منابع مشابه
Structure-based functional analysis of the replication protein of plasmid R6K: key amino acids at the pi/DNA interface.
In previous work, we characterized the bases in an iteron of plasmid R6K that are important for the binding of pi protein monomers and dimers. Here we investigate the following six amino acids of pi, encoded by pir, hypothesized to be important for DNA contact: Ser71, Try74, Gly131, Gly211, Arg225, and Arg254.
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